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anti human mouse trem2  (R&D Systems)


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    R&D Systems anti human mouse trem2
    Anti Human Mouse Trem2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+mouse+trem2+antibody/Human%2FMouse+TREM2+Alexa+Fluor%C2%AE+488-conjugated+Antibody/bio_rxiv__64898__2026__04__02__716182-287-25-29
    Average 93 stars, based on 6 article reviews
    anti human mouse trem2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: After incubation, culture medium was removed from all wells of each microplate and replaced with 20 μL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1X Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent).

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: After incubation, culture medium was removed from all wells of each microplate and replaced with 20 μLof “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1X Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent).

    Concentration Assay:

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: After incubation, removed culture medium from all wells of each microplate and replaced medium with 20 uL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1× Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent). .. Assay Buffer contained diluted test articles (in 1% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061). ..

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: After incubation, culture medium was removed from all wells of each microplate and replaced with 20 μL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1× Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent). .. Assay Buffer contained diluted test articles (in 10% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, or 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061). ..

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells2 agonists and methods of use
    Article Snippet: After incubation, removed culture medium from all wells of each microplate and replaced medium with 20 uL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1× Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent). .. Assay Buffer contained diluted test articles (in 1% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061). ..

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: After incubation, culture medium was removed from all wells of each microplate and replaced with 20 μL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1× Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent). .. Assay Buffer contained diluted test articles (in 1% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, or 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061). .. Assay Buffer contained diluted test articles (in 1% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, or 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061).

    Positive Control:

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: After incubation, removed culture medium from all wells of each microplate and replaced medium with 20 uL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1× Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent). .. Assay Buffer contained diluted test articles (in 1% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061). ..

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: After incubation, culture medium was removed from all wells of each microplate and replaced with 20 μL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1× Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent). .. Assay Buffer contained diluted test articles (in 10% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, or 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061). ..

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells2 agonists and methods of use
    Article Snippet: After incubation, removed culture medium from all wells of each microplate and replaced medium with 20 uL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1× Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent). .. Assay Buffer contained diluted test articles (in 1% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061). ..

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: After incubation, culture medium was removed from all wells of each microplate and replaced with 20 μL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1× Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent). .. Assay Buffer contained diluted test articles (in 1% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, or 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061). .. Assay Buffer contained diluted test articles (in 1% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, or 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061).

    Negative Control:

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: After incubation, removed culture medium from all wells of each microplate and replaced medium with 20 uL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1× Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent). .. Assay Buffer contained diluted test articles (in 1% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061). ..

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: After incubation, culture medium was removed from all wells of each microplate and replaced with 20 μL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1× Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent). .. Assay Buffer contained diluted test articles (in 10% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, or 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061). ..

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells2 agonists and methods of use
    Article Snippet: After incubation, removed culture medium from all wells of each microplate and replaced medium with 20 uL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1× Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent). .. Assay Buffer contained diluted test articles (in 1% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061). ..

    Article Title: Heterocyclic compounds as triggering receptor expressed on myeloid cells 2 agonists and methods of use
    Article Snippet: After incubation, culture medium was removed from all wells of each microplate and replaced with 20 μL of “Assay Buffer” comprised of DMEM-F12 (Corning 10-092-CM) supplemented with 1× Penicillin/Streptomycin (Corning 30-002-CI) and 0.1% Pluronic F-68 Polyol (MP Biomedical 092750049) using a Bravo 384-well pipette-based liquid handling system (Agilent). .. Assay Buffer contained diluted test articles (in 1% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, or 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061). .. Assay Buffer contained diluted test articles (in 1% final DMSO concentration for compounds) or 100 nM anti-human/mouse TREM2 antibody (R&D Systems MAB17291) as a positive control, or 100 nM or rat IgG2B isotype Ab as a negative control (R&D Systems MAB0061).

    Activation Assay:

    Article Title: Anti-human TREM2 antibodies and uses thereof
    Article Snippet: .. Several of the antibodies are more potent and produce a greater level of maximum activation of TREM2/DAP12 signaling than a commercially available rat anti-human/mouse TREM2 antibody (mAb17291; Rat IgG2b Clone #237920, R&D Systems), which had an EC50 value of 0.50 nM and an Emax of 13.8 in this assay. .. Several of the antibodies are more potent and produce a greater level of maximum activation of TREM2/DAP12 signaling than a commercially available rat anti-human/mouse TREM2 antibody (mAb17291; Rat IgG2b Clone #237920, R&D Systems), which had an EC50 value of 0.50 nM and an Emax of 13.8 in this assay.

    Western Blot:

    Article Title: Anti-human TREM2 antibodies and uses thereof
    Article Snippet: .. A commercially available rat anti-human/mouse TREM2 antibody (mAb17291; Rat IgG2b Clone #237920, R&D Systems) increased pSyk levels in both R47H and wild-type BMDMs as measured by Western Blot with the effect being more pronounced in the wild-type BMDMs (FIG. 9A). .. A commercially available rat anti-human/mouse TREM2 antibody (mAb17291; Rat IgG2b Clone #237920, R&D Systems) increased pSyk levels in both R47H and wild-type BMDMs as measured by Western Blot with the effect being more pronounced in the wild-type BMDMs (FIG. 9A).



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    Image Search Results


    (A, C). Representative immunofluorescence images of the medial corpus callosum in the brain and the dorsal region in the lumbar spinal cord stained with antibodies for MerTK and TREM2. (B, D) Mean intensities graphed to depict the comparison between brain and spinal cord. Mean intensities were obtained using Fiji Image J software. Error bars represent standard error of the mean. Statistical analysis was performed using a 3-way ANOVA with Tukey’s post-hoc analysis, n = 5 – 15 where each sample represents a different animal, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p <0.0001.

    Journal: bioRxiv

    Article Title: Spinal Cord Microglia Exhibit Impaired Repair Responses to Myelin Damage

    doi: 10.64898/2026.02.23.707565

    Figure Lengend Snippet: (A, C). Representative immunofluorescence images of the medial corpus callosum in the brain and the dorsal region in the lumbar spinal cord stained with antibodies for MerTK and TREM2. (B, D) Mean intensities graphed to depict the comparison between brain and spinal cord. Mean intensities were obtained using Fiji Image J software. Error bars represent standard error of the mean. Statistical analysis was performed using a 3-way ANOVA with Tukey’s post-hoc analysis, n = 5 – 15 where each sample represents a different animal, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p <0.0001.

    Article Snippet: The resulting cells were incubated in an FC block solution (Tonbo 70-0161, 1:100) for 10 minutes and then stained in an antibody mix for 30 minutes which consisted of CD45 violetFluor 450 (Tonbo 75-0451, 1:75), CD11b PerCP-Cyanine5.5 (Tonbo 65-0112, 1:75), Brilliant Violet 605 anti-mouse CD16 (Biolegend 158027, 1:75), FITC anti-mouse CD68 (Biolegend 137006, 1:75), APC/Fire 810 anti-mouse CX3CR1 (Biolegend 149054, 1:75), APC anti-mouse F4/80 (Biolegend 123116, 1:75), APC/Fire 750 anti-mouse CD73 (Biolegend 127221, 1:75), PE anti-mouse TREM2 (R&D Systems FAB17291P, 1:75), and viability dye (Tonbo 13-0871, 1:1000).

    Techniques: Immunofluorescence, Staining, Comparison, Software